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2 edition of action of a mast cell proteinase on prothrombin and factor X. found in the catalog.

action of a mast cell proteinase on prothrombin and factor X.

Alastair Robert George Wylie

action of a mast cell proteinase on prothrombin and factor X.

by Alastair Robert George Wylie

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Published .
Written in English


Edition Notes

Thesis (Ph.D.)--The Queen"s University of Belfast, 1978.

The Physical Object
Pagination1 v
ID Numbers
Open LibraryOL19318167M

  Chronic urticaria (CU) is a widespread skin disease characterized by the recurrence of transient wheals and itch for more than 6 weeks. It is believed that over 50% of CU cases are accompanied by a deep subcutaneous and/or submucosal edema, called angioedema (AE; 1). It is well known that the characteristic symptoms of urticaria appear following the activation of mast cells Cited by: Human prothrombin is prepared from fresh frozen human plasma as described by Bajaj and coworkers (2). Bovine prothrombin is prepared from fresh bovine plasma using a modification of the procedure described by Owen and coworkers (3). Purified prothrombin is supplied in 50% (vol/vol) glycerol/H2O and should be stored at oC.

  FACTOR II /PROTHROMBIN Vitamin K-dependent serine protease. Prothrombin FACTOR Xa Thrombin. Soluble fibrinogen THROMBIN Insoluble fibrin. • Activates factors V, VIII, XI and XIII. • Thrombin along with thrombomodulin present on endothelial cell surfaces form a complex that converts protein C to activated protein C (APC). Common Pathway. In the final common pathway, prothrombin is converted to thrombin. When factor X is activated by either the intrinsic or extrinsic pathways, it activates prothrombin (also called factor II) and converts it into thrombin using factor V. Thrombin then cleaves fibrinogen into fibrin, which forms the mesh that binds to and strengthens the platelet plug, finishing coagulation and.

luminal antigen challenge (8,9). Mast cells contain numerous chemical mediators (10,ll). Recent work (12) has described the systemic release of a protease, rat mast cell protease (RMCP) II, that is specific for mucosal mast cells during degranulation in response to repeated exposure parasitic antigens in rats.   Action of activated Factor X to form prothrombin activator. activated Factor X combines with Factor V and platelet or tissue phospholipids to form the complex called prothrombin activator. Role of Calcium - Except for the first two steps in the intrinsic pathway, calcium ions are required for promotion or acceleration of all the blood.


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Action of a mast cell proteinase on prothrombin and factor X by Alastair Robert George Wylie Download PDF EPUB FB2

The action of a mast cell proteinase on prothrombin and factor X. Author: Wylie, A. ISNI: X Awarding Body: Queen's University Current Institution: Queen's University Belfast Date of Award: Availability of Full Text.

Proteolysis of bovine and human prothrombin and of bovine factor X by rat mast cell proteinase. Wylie AR, Lonsdale-Eccles JD, Blumsom NL, Elmore DT.

Thromb Res, 44(3), 01 Nov Cited by: 2 articles | PMID: Cited by: 2. The alpha-chymotrypsin-like proteinase from rat peritoneal mast cells (RMCP I) rapidly destroyed the normal clotting activity of purified, calcium-free, bovine prothrombin, human prothrombin and bovine factor X and simultaneously removed similar N-terminal peptides (Mr approximately 5,) from both prothrombin and the 'light' chain of factor by: 3.

MC T mast cells express only tryptases (α and β), whereas MC TC mast cells express all three types of mast cell-specific proteases. Murine mast cells are also classified by their protease constituents, being subdivided into the connective tissue (CTMC) and mucosal (MMC) subtypes in this by: The α-chymotrypsin-like proteinase from rat peritoneal mast cells (RMCP I) rapidly destroyed the normal clotting activity of purified, calcium-free, b Cited by: 3.

Human mast cells contain proteases that are important functional components and serve as markers of mast cell activation or degranulation. Although tryptase is the best recognized mast cell protease, chymase and Cathepsin G also are found in some human mast by: 6. Tryptase, released upon mast cell degranulation, is the possible candidate for triggering PAR2 activation.

Effector cell protease receptor 1 (EPR-1), factor Xa and inflammation Factor Xa is a protease of the coagulation cascade that medi-ates the conversion of prothrombin to thrombin.

Interest-ingly, factor Xa also activates PAR2 and, by binding to. This cascade results in the activation of factor X. Activated factor X is an enzyme that converts prothrombin to thrombin. Thrombin converts fibrinogen to fibrin monomers, which then polymerize in fibrin fibers.

Fibirin fibers form a losse meshwork that is stabilized by crosslinks created by factor XIII. The stabilized meshwork of fibrin fibers ins now a clot that traps red blood cells and platelets and.

Cathepsin G, isolated from human polymorphonuclear leukocytes, was found to effect rapid and specific degradation and biological inactivation of bovin Cited by: Inactivation of coagulation proteins.

Plasma protease inhibitors; Failure of plasma protease inhibitor systems Thrombus forms a fibrin network longer tail entraps red cells) Fibrin tail may detach forming emboli which may travel to pulmonary arteries Prothrombin (factor II). x; UniProtKB. Protein knowledgebase.

UniParc. Sequence archive. Help. Mast cell protease 2 (EC: Mucosal mast cells. Gene expression databases. Bgee i: ENSMUSG Expressed in mucous cell of stomach and 32 other tissues ExpressionAtlas i: P baseline and.

A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Previous studies from our laboratory have shown that during angiogenesis in vitro, rmMCP-7 (recombinant mouse mast cell protease-7) stimulates endothelial cell spreading and induces their penetration into the matrix.

The ability of rmMCP-7 to induce angiogenesis in vivo was assessed in the present study using a directed in vivo angiogenesis assay (DIVAA™).Author: Devandir A. de Souza Junior, Carolina Santana, Gabriel V.

Vieira, Constance Oliver, Maria Celia Jamu. The alpha-chymotrypsin-like proteinase from rat peritoneal mast cells (RMCP I) rapidly destroyed the normal clotting activity of purified, calcium-free, bovine prothrombin, human prothrombin and.

cells Article Mast Cell Protease 7 Promotes Angiogenesis by Degradation of Integrin Subunits Devandir A. de Souza Junior, Carolina Santana, Gabriel V. Vieira, Constance Oliver and Maria Celia Jamur * Department of Cell and Molecular Biology and Pathogenic Author: Devandir A.

de Souza Junior, Carolina Santana, Gabriel V. Vieira, Constance Oliver, Maria Celia Jamu. Effector cell protease receptor 1 (EPR-1), factor Xa and inflammation Factor Xa is a protease of the coagulation cascade that mediates the conversion of prothrombin to thrombin.

Interestingly, factor Xa also activates PAR2 and, by binding to effector cell protease receptor 1 (EPR-1), participates in several cellular events that are involved in Cited by: The net result is formation of a complex of activated substances collectively called prothrombin activator 2)The prothrombin activator catalyzes conversion of prothrombin into thrombin 3) **Thrombin acts as an enzyme to convert fibrinogen into fibrin fibers that enmesh platelets, blood cells, and plasma to form the clot.

Proteinase-activated receptors (PARs, nomenclature as agreed by the NC-IUPHAR Subcommittee on Proteinase-activated Receptors []) are unique members of the GPCR superfamily activated by proteolytic cleavage of their amino terminal t proteinase-induced hydrolysis unmasks a tethered ligand (TL) at the exposed amino terminus, which acts intramolecularly at the binding site in the.

then removing all the cells, and the clot, by centrifugation. Several of the clotting proteins are absent in serum, Prothrombin* Factor II Protease zymogen (plasma) Fibrinogen Factor I Fibrin precursor (plasma, platelets) its proteolytic action on factor X being shown here by the open blue arrow.

Any species shown beside the open enzyme File Size: KB. Once factor X has been activated by either the intrinsic or extrinsic pathway, the enzyme prothrombinase converts factor II, the inactive enzyme prothrombin, into the active enzyme thrombin.

(Note that if the enzyme thrombin were not normally in an inactive form, clots would form spontaneously, a condition not consistent with life.).

Factor X, also known by the eponym Stuart–Prower factor, is an enzyme of the coagulation cascade. It is a serine endopeptidase. Factor X is synthesized in the liver and requires vitamin K for its synthesis.

Factor X is activated, by hydrolysis, into factor Xa by both factor IX and factor VII with its cofactor, tissue factor. It is therefore the first member of the final common pathway or thrombin pathway. It Aliases: F10, FX, FXA, coagulation factor X.Prothrombin fragment F is formed by the action of which protease action on prothrombin.

Factor Xa. Clinical conditions associated with DIC include. Snake bites, sepsis, Acute promyelocytic leukemia. What laboratory test is diagnostic of DIC. Prolonged thrombin time, Elevated D dimers, hypofibrinogenemia.

Thrombin is a serine protease that is formed from its circulating precursor, prothrombin, by the action of activated factor X(prothrombin activator). Factor X can be activated by reactions in either of two systems, an intrinsic and an extrinsic system.